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In vitro neurotoxicity using human iPSC-derived neuronal models


The urgent need for rapid in vitro approaches based on human cells prompted Tukker et al. to investigate different hiPSC-derived neuronal models for neurotoxicity testing. The cell models differed in the ratios of excitatory and inhibitory neurons and the presence or absence of astrocytes. All models over developed functional networks that exhibit spontaneous neuronal activity. Subsequent neurotoxicity assessment demonstrates that these cultures can be modulated with known seizurogenic compounds, such as picrotoxin (PTX) and endosulfan, and the neurotoxicant methylmercury (MeHg), although the chemical-induced effects depend on model characteristics. As such, these results highlight the need for thorough model characterization prior to neurotoxicity screening (NeuroToxicology).